Effects of in vivo phlorizin treatment and in vitro addition of carnitine, propionate, acetate, and 5-tetradecyloxy-2-furoic acid on palmitate metabolism in ovine hepatocytes

Modulatory effects of l-carnitine, acetate, propionate, and 5-tetradecyloxy-2-furoic acid (TOFA; an inhibitor acetyl-CoA carboxylase) on oxidation esterification [1-14C]-palmitate were studied in hepatocytes isolated from phlorizin-treated control wethers. Our hypotheses that (1) palmitate would be greater sheep injected with phlorizin; (2) l-carnitine increase more (3) acetate propionate decrease partly through action carboxylase. Palmitate metabolism did not differ between cells those Carnitine increased to CO2 acid-soluble products (ASP; mainly ketone bodies) decreased both groups wethers, but the ASP was Propionate wethers total esterified triglyceride, effect larger Acetate blunted affect oxidation. Addition TOFA production triglyceride presence propionate. The lack interaction indicates does inhibit carnitine palmitoyltransferase I via cytosolic generation methylmalonyl-CoA by In conclusion, although vivo phlorizin treatment vitro ovine hepatocytes, stimulatory inhibitory ASP. Metabolism carboxylase or esterification. Results provide additional insight into fatty hepatocytes.